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Flame photometry


Flame photometry

Model 420 – Dual Channel Flame Photometer
The manufacturer: Sherwood Scientific Ltd., UK

  • Recomended method of HKMB for determination of electrolytes
  • The determination of Sodium, Potassium and Lithium in serum, urine and plasma
  Measured ranges CV
Serum Na: 100.0 – 199.9 mmol/L 0.5% (diluted 1 in 200)
  K: 1.00   -  9.99   mmol/L 1.0% (diluted 1 in 200)
  Li: 1.00   -  5.00   mmol/L 1.5% (diluted 1 in 50)
Urine Na: 100.0 – 199.0 mmol/L 1.0% (diluted 1 in 5 prior to 1 in 200)
  K:  40.0  -   120.0 mmol/L 0.5% (diluted 1 in 5 prior to 1 in 200)
  • The linearity of Na and K is +/- 2 % over all measuring range
  • Minimum Sample volume:
    Manual dilution: 30 – 60 µL
    Dilutor dilution: 60 – 120 µL
    Autosampler: 0.5 – 1.8 mL
  • Throughput: ~ 60 samples per hour
  • Automatic ignition and optimisation of the flame conditions (Propane or Butane only)
  • Optional automatic sample dilution using continuous flow Diluter
  • Sample dilution for the measurement of Na/K 1:200
  • Razrjeđenje uzorka za mjerenje Li 1:50
  • Throughput for Autosampler: ~ 60 samples per hour
  • Precision and accuracy of the measurement are much improved by the use of a Lithium Internal Standard which compensates for fluctuation in flame conditions and dilution errors (when continuous flow diluter used).
  • The possibility of data transfer and storage to the computer over RS-232.